Application Note: Anti-Glucose Oxidase has been tested in western blot and is suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
Concentration Value: 1 mg/ml
ELISA Dilution: 1:2,000 - 1:10,000
Immunohistochemistry Dilution: User Optimized
Western Blot Dilution: 1:1000 - 1:5000
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Immunogen: Glucose Oxidase [Aspergillus niger]
Physical State: Liquid (sterile filtered)
Purity and Specificity: Anti-Glucose Oxidase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger]. Cross reactivity against Glucose Oxidase from other tissues and species may occur but have not been specifically determined.
Background: Glucose Oxidase enzyme is widely applied for the determination of glucose in body fluids and in removing residual glucose or oxygen from foods and beverages. Furthermore, glucose oxidase-producing molds such as aspergillus and penicillium species are used for the biological production of gluconic acid.
Low Endotoxin: No
Other: User Optimized
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