Conjugation: Biotin
Description of Target: MOUSE IgG2a NEGATIVE CONTROL:Biotin
Immunogen: Activated rat T-helper cells.
Key Reference: 1. Avigdor, A. et al. (2004) CD44 and hyaluronic acid cooperate with SDF-1 in the trafficking of human CD34+ stem/progenitor cells to bone marrow. Blood. 103: 2981-2989.
Product Format: Liquid. Phosphate buffered saline
Purification: Purified IgG prepared by affinity chromatography from tissue culture supernatant
Specificity#: This product is suitable for use as a negative control reagent for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a to human tissue.
This antibody may not be suitable for intracellular staining on some cell types.
Clone MRC OX-34 recognises a rat cell surface marker, and therefore cannot be used as a negative control in this species.
This product is routinely tested in flow cytometry on rat splenocytes to confirm antibody activity and on human whole blood to test for suitability as a negative control.
Test results have shown that This product is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.