Application Note: WB: 0.1-0.5 μg/mL. IHC-P: 0.5-1 μg/mL. IHC-Fr: 0.5-1 μg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Calculated MW: 40
Form: Liquid
Buffer (with preservative): 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Concentration: 0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Background: This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2011]
Uniprot ID: O95388
Antigen Species: Human
Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human WISP1, different from the related mouse and rat sequences by one amino acid.
Purification: Purified by antigen-affinity chromatography
Conjugation: Unconjugated
Full Name: cellular communication network factor 4
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