Clone Number: SQab1721
Immunogen: Synthetic peptide around the C-terminus of Vimentin
Concentration: n/a
Format: Affinity purification with immunogen.
Storage buffer: PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Additional info: Application note: IHC-P: Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0 IHC-Fr: guaranteed based on publication PMID: 35011683 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.Storage instruction: For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.Background: Vimentin is a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
Applications: FC, ICC/IF, IHC-fr, IHC-P, IP, WB
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