Application Note: WB: 1:2,000-1:10,000. ELISA: 1:20,000-1:100,000. IHC: 1:1,000-1:5,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Specificity/Sensitivity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey Serum, Sheep IgG and Sheep Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins.
Form: Liquid
Buffer (with preservative): 0.02M Potassium Phosphate, 0.15M NaCl, 1% BSA, 0.01% Sodium azide.
Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species: Sheep
Immunogen: Sheep IgG whole molecule
Target Isotype: IgG
Conjugation: Biotin