Product Description: EasyBlot anti-Rabbit IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of rabbit IgG and does not bind the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP). EasyBlot is simple to use and results in Western Blot detection of only the target band in your IP experiments. Exercise caution when using Protein A/G for IP with EasyBlot. Protein A/G contaminants bind to rabbit IgG with high affinity and may cause contaminating bands with a molecular weight similar to IgG heavy chain or light chian.
To minimize background from Protein A/G contamination, we strongly recommend the use of EasyBlot Kit anti-Rabbit IgG (Optimized for Prot A/G) (GTXGTX225856-01) or EasyBloker (GTX425858) to minimize protein A/G contamination or you can use anti-Rabbit IgG Agarose beads (GTX27019) instead of Protein A/G.
Application Note: WB: 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Positive Control: native-rabbit IgG
Form: Liquid
Buffer (with preservative): 0.05M Tris, 0.15M NaCl, 1%BSA, 40% Glycerol, 0.025% ProClin 300.
Concentration: 0.12 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species: Rabbit
Target Isotype: IgG
Purification: Affinity purified by Protein G.
Conjugation: HRP
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