Clone: NI 69 (A89-034) and NI 184 (A89-035)
Specificity: Human IgA, isotype specific.
Antibody Modification: Unconjugated
Presentation: Lyophilized
Immunogen: Highly purified monoclonal IgA isolated from pooled human serum.
Resuspension Liquid: Reconstitute with distilled water.
Gene: Human IgA
Description: Human IgA antibody LS-B16433 is an unconjugated mouse monoclonal antibody. Validated for ELISA, IF and IHC. Tested on 20 paraffin-embedded human tissues.
Usage: The reactivity of the antiserum is restricted to determinants on the CH2 domain of IgA. It reacts with both subclasses of IgA as tested in hemagglutination, hemagglutination inhibition, indirect binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgA, hemagglutination and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200. in ELISA from 1:500 upwards. in Western blotting from 1:1000 upwards. These data should be interpreted as general recommendations only.
Recommended Storage: Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
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