Formulation: 50 mM Tris HCl, pH 8.0, with 150 mM sodium chloride, and 10% glycerol
Purity: ≥90% estimated by SDS-PAGE
Formula Weight: 0
Shelf life (days): 365
Notes: Biliverdin reductase B, also known as S-nitroso-CoA-assisted nitrosyltransferase (SCAN), is an NADPH-dependent reductase and S-nitroso-CoA-dependent nitrosyltransferase encoded by BLVRB in humans that has roles in heme metabolism and insulin signaling.{68929,71121} It is a monomeric enzyme composed of a dinucleotide-binding domain that binds both NADPH and a substrate.{68929} It is expressed in the liver and in erythrocytes and localizes to the cytoplasm.{68930,71121} Biliverdin reductase B uses NADPH as an electron donor to reduce biliverdin-IXβ to bilirubin in the last step of heme degradation during fetal development.{68929,68931} It can also reduce biliverdin-IXγ and biliverdin-IXδ, but not biliverdin-IXα, which is the predominant isoform in adults, as well as flavins, such as flavin mononucleotide (FMN), and ferric iron. Using S-nitroso-CoA (SNO-CoA) as a cofactor, biliverdin reductase B transfers SNO to cysteine residues in target proteins, such as the insulin receptor (InsR) and insulin receptor substrate 1 (IRS-1), which reduces insulin signaling.{71121} Human skeletal muscle, visceral adipose, and subcutaneous adipose levels of biliverdin reductase B are positively associated with increased SNO-InsRβ levels in these tissues and with body mass index (BMI). Intratumoral levels of biliverdin reductase B are increased in comparison with levels in non-cancerous tissue in patients with esophageal carcinoma, endometrial carcinoma, or pancreatic cancer, among other cancers.{68932} A serine-to-leucine substitution at residue 111 of biliverdin reductase B inhibits its activity for both biliverdins and flavins and is a risk factor for thrombocytosis in humans.{68933} Cayman’s Biliverdin Reductase B (human, recombinant) protein can be used for ELISA, enzyme activity assay, and Western blot applications.
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