Product Description: Bis-Tris is an amine buffer suitable for protein and nucleic acid systems with a pH buffer range of 5.8-7.2. Bis-Tris can also be mixed with HEPES and cacodylic acid buffer to create a pH 8 environment and monitor fluorescence emission intensity at 305 nm. Gels formulated with Bis-Tris also avoid protein degradation in samples prepared at higher pH (pH 8.5)[1][2][3].
Formula: C8H19NO5
References: [1]Williams AS, et al. Domain 4 of the anthrax protective antigen maintains structure and binding to the host receptor CMG2 at low pH. Protein Sci. 2009 Nov;18(11):2277-86./[2]Hachmann JP, et al. Models of protein modification in Tris-glycine and neutral pH Bis-Tris gels during electrophoresis: effect of gel pH. Anal Biochem. 2005 Jul 15;342(2):237-45./[3]Oh, Soon Jin, et al. Structure and phosphodiesterase activity of Bis-Tris coordinated lanthanide(III) complexes.
CAS Number: 6976-37-0
Molecular Weight: 209.24
Compound Purity: 99.91
Research Area: Others
Solubility: H2O : 250 mg/mL (ultrasonic)
Target: Biochemical Assay Reagents
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