Formulation: 5 ml of cysteinyl leukotriene affinity sorbent
Shelf life (days): 365
Notes: Traditional purification of urine and plasma samples prior to measurement by ELISA has required the use of reverse phase C-18 columns and various organic solvents. As this method relies upon polarity for separation, the final purified product is a non-specific mixture of many compounds. Further purification by TLC or HPLC is then required to isolate a single product. As an alternative, affinity chromatography provides a specific, reliable, and convenient means of purification for a variety of sample types. Cayman Chemical has developed an affinity sorbent for the purification of cysteinyl leukotriene (cysLT) from urine and plasma samples. Each vial of sorbent contains 5 ml of cysLT affinity sorbent (mouse anti-cysLT covalently bound to Sepharose 4B).
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