Formulation: 50 mM HEPES, pH 7.8, with 150 mM sodium chloride and 10% glycerol
Purity: ≥60% estimated by SDS-PAGE
Shelf life (days): 180
Notes: Nucleophosmin is a nucleolar phosphoprotein that is involved in diverse biological processes, including ribosome maturation, DNA repair, and mitotic spindle assembly.{69419} It is highly post-translationally modified and composed of an oligomerization domain, which contains two nuclear export signals, three acidic domains, an intrinsically disordered region, which contains two nuclear localization signals, and a C-terminal RNA-binding domain.{69419,69420} Nucleophosmin is ubiquitously expressed and shuttles between the nucleolus and cytoplasm; however, alternative splicing of NPM1 produces a variant that lacks the RNA-binding domain and nucleolar localization signal and is instead localized throughout the nucleus.{69421,69419,69420} Nucleophosmin has histone- and protein chaperone activity and plays a role in ribosome assembly and export, centrosome duplication, cell cycle control, the stress response, and embryogenesis.{69419} Nucleophosmin citrullinated by PAD4 increases p53 stability and radiation sensitivity in a thyroid cancer cell line exposed to radiation.{69422} This product contains purified nucleophosmin (Item No. 37621) that has been modified with PAD4, which is subsequently depleted by affinity chromatography.
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