Formulation: 100 mM Tris-HCl, pH 8.0, with 5 mM EGTA, 1mM calcium chloride, and 30% glycerol
Purity: Clarified lysate
Shelf life (days): 180
Notes: 5-Lipoxygenase (5-LO) is an enzyme encoded by ALOX5 in humans that is involved in leukotriene biosynthesis.{53405} It is composed of an N-terminal regulatory domain and a C-terminal catalytic domain and is primarily expressed in leukocytes. Increases in intracellular calcium levels or cellular stress induce translocation of 5-LO from the cytosol or nucleoplasm, depending on the cell type and 5-LO phosphorylation status, to the nuclear envelope, where it interacts with 5-LO-activating protein (FLAP), which transfers arachidonic acid (Item Nos. 90010 / 90010.1 / 10006607) to 5-LO.{53405,53406} 5-LO catalyzes the conversion of arachidonic acid to 5(S)-HpETE (Item No. 44230) and then to leukotriene A4 (LTA4).{53407} Other substrates of 5-LO include 5,8,11,14,17-eicosapentaenoic acid, 5,8,11-eicosatrienoic acid, 5,8-eicosadienoic acid, 12-HpETE, and 15-HpETE. Alox5 knockout mice are protected against arthritis and pulmonary inflammation.{53406} Knockout of Alox5 also protects apolipoprotein E-deficient hyperlipidemic mice from high-fat diet-induced hepatic injury and inflammation.{53408} Levels of 5-LO are elevated in postmortem hippocampus and cortex of patients with Alzheimer's disease.{53409} Cayman's 5-Lipoxygenase (human, recombinant) can be used for enzyme activity assays.
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