Formulation: 50 mM Tris-HCl, pH 7.5, 200 mM sodium chloride, and 20% glycerol
Formula Weight: 0
Shelf life (days): 365
Notes: Cyclin-dependent kinase 7 (Cdk7) is a CDK-activating kinase (CAK) that is mainly involved in cell cycle progression and gene transcription, but also has a role DNA repair.{59281,51862} It is composed of an N-terminal domain that binds cyclin H and a C-terminal domain that binds the CAK assembly factor MAT1 and putative nuclear localization sequences at the N- and C-termini.{51862} Cdk7 is ubiquitously expressed and localizes to the nucleus.{51863} In the context of cell cycle progression, Cdk7, in the CAK complex with cyclin H and MAT1, phosphorylates other CDKs such as Cdk1/cyclin A and Cdk2/cyclin E to activate the cell cycle.{51862} Cdk7 is also a component of the transcription factor IIH (TFIIH) complex where it activates RNA polymerase II directly via phosphorylation, which influences transcription initiation, or indirectly through activation of Cdk9, which promotes elongation. Knockout of CDK7 or inhibition of Cdk7 inhibits breast cancer proliferation in vitro and tumor growth in vivo.{31733} The intratumoral expression of CDK7 is increased in patients with oral squamous cell carcinoma (OSCC) and is associated with increased tumor grade and poor prognosis.{51864} Cayman’s Cdk7 (human, recombinant) protein has a calculated molecular weight of 65.6 kDa.
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