Formulation: 50 mM HEPES, pH 8.0, with 150 mM sodium chloride
Purity: ≥70% estimated by SDS-PAGE
Shelf life (days): 365
Notes: Cyclic dinucleotide phosphodiesterase (CdnP) is a soluble phosphodiesterase involved in regulating cyclic dinucleotide signaling during intracellular infections of M. tuberculosis.{36095} It is composed of an N-terminal Asp-His-His (DHH) domain and a C-terminal DHH-associated (DHHA1) domain that catalyze the hydrolysis of cyclic dinucleotides. CdnP hydrolyzes a variety of cyclic dinucleotides, including cyclic di-AMP (Item No. 17753), cyclic di-GMP (Item No. 17144), and 2′3′-cGAMP (Item No. 19887), which decreases their recognition by the stimulator of interferon genes (STING), a key mediator in the host innate immune response.{36905,33472,36906} Knockout of the gene encoding CdnP, CnpB, increases the production of IFN-β in isolated mouse bone marrow-derived macrophages (BMDMs) infected with CnpB-/- M. tuberculosis, as well as decreases pulmonary and splenic bacterial burden and increases survival in CnpB-/- M. tuberculosis-infected mice.{36097} Cayman’s CdnP (Mycobacterium tuberculosis strain ATCC 25618H37Rv recombinant) protein contains N-terminal Trx- and His-tags followed by a thrombin cleavage site and an S-tag followed by an enterokinase cleavage site.
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