Formulation: Lyophilized from sterile 50 mM Tris, pH 8.0, 100 mM sodium chloride, and 10% glycerol
Purity: ≥97% estimated by SDS-PAGE
Shelf life (days): 365
Notes: Coagulation Factor X is a vitamin K-dependent plasma serine protease involved in the intrinsic, extrinsic, and common pathways of the blood coagulation cascade.{72150,52856} It is composed of an N-terminal light chain that contains a signal sequence, a propeptide, carboxylated glutamic acid residues (Gla domain), and two EGF domains and a C-terminal heavy chain that contains the activation peptide and a chymotrypsin-like catalytic domain.{72150} Factor X is produced as an inactive precursor, which is activated to Factor Xa following signal peptide cleavage, glycosylation, and further cleavage by the Factor VIIIa-IXa or tissue factor-FVIIa complexes of the intrinsic or extrinsic pathways, respectively. Factor Xa, in complex with Factor Va, converts prothrombin to thrombin. Factor Xa also cleaves severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein, also known as surface glycoprotein, and prevents SARS-CoV-2 viral entry in vitro, and Factor X levels are increased in the lungs of patients with COVID-19.{72151} Knockout of F10, the gene encoding Factor X, in mice induces embryonic lethality and fatal internal bleeding of surviving neonates.{72152} Mutations in F10 result in Factor X deficiency, a rare bleeding disorder characterized by CNS and gastrointestinal bleeding, as well as hemarthroses and hematomas.{72150} Cayman’s Coagulation Factor X (human, recombinant) protein consists of 468 amino acids, has a calculated molecular weight of 52.8 kDa, and a predicted N-terminus of Asn32 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular masses are 48 and 22 kDa, corresponding to the cleaved heavy and light chains, respectively, due to glycosylation.
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