Formulation: 50 mM Tris, pH 8.0, containing 1 mM DTT and 10% glycerol
Purity: ≥85% estimated by SDS-PAGE
Shelf life (days): 365
Notes: Flap endonuclease 1 (FEN1), a RAD2 Class II nuclease, plays a crucial role in DNA repair, replication, and genomic stability.{28481} FEN1 removes the 5'-unannealed flap during Okazaki fragment processing in lagging strand DNA synthesis.{28483} Removal of the DNA flap is also critical in long-patch base excision repair and regulation of recombination.{22126} In yeast, FEN1 mutants display sensitivity to UV irradiation, deficient chromosome segregation, and cell cycle arrest in S phase.{28482} Furthermore, haploinsufficiency of FEN1 in mice leads to rapid tumor progression.{28482} FEN1 is overexpressed in many forms of cancer, including lung, gastric, prostate, pancreatic, brain, and breast.{22126}
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