Formulation: Lyophilized from sterile 50 mM Tris, pH 8.0, with 10% glycerol
Purity: ≥85% estimated by SDS-PAGE
Shelf life (days): 365
Notes: Fragile histidine triad diadenosine triphosphatase (FHIT) is a hydrolase and tumor suppressor.{60935,60936,60937} It contains a His-x-His-x-His motif that hydrolyzes diadenosine triphosphate (Ap3A), as well as Ap4A and ATP.{60935} FHIT is expressed in spleen, brain, kidney, lung, and liver and localizes to the plasma membrane, nucleus, mitochondria, and cytoplasm.{60938,60939,60940} It is involved in tumor suppression and genome stability.{60936} Overexpression of FHIT induces apoptosis in lung cancer cells and reduces tumor growth in lung cancer mouse xenograft models.{60941} Homozygous or heterozygous loss of FHIT expression is prevalent in several cancers, and the FHIT gene contains a common fragile site, FRA3B, that is a translocation breakpoint associated with hereditary kidney cancer.{60937} Cayman’s FHIT (human, recombinant) protein consists of 153 amino acids and has a calculated molecular weight of 17.7 kDa. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is 18 kDa.
English
Deutsch
