Formulation: 50 mM potassium phosphate, pH 7.8, with 200 mM sodium chloride, 10% glycerol, and 0.1 mM TCEP
Purity: ≥70% estimated by SDS-PAGE
Shelf life (days): 365
Notes: Ferroptosis suppressor protein 1 (FSP1), also known as apoptosis-inducing factor mitochondria-associated 2 (AIFM2), is a flavoprotein and NAD(P)H-dependent oxidoreductase that inhibits ferroptosis in a glutathione peroxidase 4- and glutathione-independent manner.{50700,50699,45864} It is encoded by AIFM2 in humans and is composed of a short N-terminal hydrophobic region followed by a flavin adenine dinucleotide-dependent oxidoreductase domain.{50699} FSP1 localizes to the plasma membrane in a myristoylation-dependent manner, where it reduces coenzyme Q10 (CoQ10; Item No. 11506) to CoQ10H2. Reduced CoQ10 functions as a radical-trapping antioxidant and inhibits lipid peroxidation. AIFM2 expression positively correlates with resistance to glutathione peroxidase 4 (GPX4) inhibitors, including (1S,3R)-RSL3 (Item No. 19288), ML-210 (Item No. 23282), and ML-162 (Item No. 20455) in cancer cell lines. Withdrawal of the ferroptosis inhibitor ferrostatin-1 (Item No. 17729) reduces tumor growth in an Fsp1/Gpx4 double knockout, but not a Gpx4 single knockout, H460 lung cancer mouse xenograft model, indicating that FSP1 confers resistance to ferroptotic cell death. Cayman's FSP1 (human, recombinant) protein can be used for enzyme assay applications.
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