Formulation: Lyophilized from sterile 40 mM Tris-HCl, pH 8.0, with 110 mM sodium chloride, 2.2 mM potassium chloride, 3 mM DTT, 0.04% Tween 20, and 20% glycerol
Purity: ≥71% estimated by SDS-PAGE
Shelf life (days): 180
Notes: JAK2 is a non-receptor tyrosine kinase that has roles in immune signaling.{19346,54923} It is composed of N-terminal FERM and SH2 domains, a regulatory pseudokinase domain, and a C-terminal kinase domain.{19346} It is widely expressed and associates with class I and class II cytokine receptors at the plasma membrane.{54921,54922} Activation of these cytokine receptors activates JAK2 and induces its dimerization and kinase activity, leading to JAK2 phosphorylation of STAT transcription factors and transcription of immune-related target genes. JAK2 signaling is inhibited by the suppressor of cytokine signaling (SOCS) proteins SOCS-1 and SOCS-3.{54924,54925} Gain-of-function mutations in JAK2 are associated with various blood disorders, including leukemias and myeloproliferative neoplasms.{54922} Cayman’s JAK2 (human, recombinant) protein can be used for enzyme activity assays.
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