Formulation: 20 mM HEPES, pH 7.4, and 150 mM sodium chloride
Purity: ≥75% estimated by SDS-PAGE
Shelf life (days): 180
Notes: Methylation of lysine residues in core histones plays a critical role in regulating gene expression.{18003} Jumonji domain containing 2E (JMJD2E) catalyzes the demethylation of histone H3 at lysince residue 9.{18004} Like other JmjC protein hydroxylase family members, JMJD2E is an α-ketoglutarate-dependent Fe (II) oxygenase.{17970} Purification of Fe-dependent JmjC family members by IMAC can result in displacement of the catalytic iron and decreased activity, therefore this Strep-tagged protein is purified by affinity chromatography using Strep-Tactin coated resin.{20824} Because of their implication in cancer cell growth, jumonji C domain-containing histone demethylase inhibitors may have the capacity to be anticancer agents.{18003}
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