Formulation: 50 mM Tris-HCl, pH 7.6, with 0.5 mM EDTA, 0.2 mM DTT, and 10% glycerol
Purity: ≥60% estimated by SDS-PAGE
Notes: Lanosterol 14-α-demethylase, also known as cytochrome P450 (CYP) isoform CYP51A1, is a heme-containing demethylase and member of the CYP450 superfamily that demethylates lanosterol (Item No. 19521) to generate sterol precursors in the synthesis of cholesterol (Item Nos. 39088 / 9003100).{24643,67752} It is ubiquitously expressed with higher levels found in the testes and ovaries and has a globular structure that is composed of a hydrophobic substrate channel, a substrate-binding cavity, and a heme-iron cofactor conjugation region.{67753,67752} Inhibition of lanosterol 14-α-demethylase decreases cholesterol levels and induces differentiation in HL-60 promyeoloblast leukemia cells.{67754} Cayman's Lanosterol 14-α-demethylase (Item No. 32928) protein can be used for ELISA, enzyme activity assay, and Western blot applications.
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