Formulation: 50 mM Tris HCl, pH 7.5, with 3 mM DTT
Purity: ≥90% estimated by SDS-PAGE
Formula Weight: 0
Shelf life (days): 365
Notes: Lipopolysaccharide α-1,4-galactosyltransferase (LgtC) is a retaining glycosyltransferase and member of glycosyltransferase family 8 (GT8) that is found in N. meningitidis, an opportunistic bacterium that can cause meningitis.{51660} It is composed of an N-terminal mixed α/β domain, which contains the active site, and a C-terminal helical domain, which is responsible for attachment to cell membranes.{51662,51661} LgtC transfers a galactosyl moiety from UDP-galactose (UDP-Gal) to the terminal lactose moiety on lipooligosaccharides (LOSs) while retaining the configuration of the donor sugar glycosidic bond.{51661} Isolated LgtC has been used in the analysis of newborn dried blood spots as a potential screening assay for galactosemia, an inborn error of metabolism characterized by a deficiency in galactose-1-phosphate uridylyltransferase (GALT), the enzyme that converts UDP-glucose to UDP-galactose.{45074} Cayman’s LgtC (N. meningitidis) protein can be used for biocatalysis and enzyme activity assay applications.
English
Deutsch
