Formulation: 50 mM Tris Hydrochloric acid, pH 7.5, with 50 mM sodium chloride, and 5 mM DTT
Purity: ≥90% estimated by SDS-PAGE
Formula Weight: 0
Shelf life (days): 365
Notes: Lipopolysaccharyl β-1,3-N-acetylgalactosaminyltransferase (LgtD) is an inverting N-acetylgalactosaminyltransferase and a member of glycosyltransferase family 2 (GT2) that is found in H. influenzae.{66905,66906} A similar protein, lipopolysaccharide α-1,4-galactosyltransferase (LgtC) that is found in N. meningitidis, is composed of an N-terminal mixed α/β domain, which contains the active site, and a C-terminal helical domain, which is responsible for attachment to cell membranes.{43832,51661} LgtD transfers an N-acetylgalactosamine from UDP-N-acetylgalactosamine (UDP-GalNAc) onto globotriose in the biosynthesis of the glycosphingolipid antigen globohexasaccharide (globoH) or globotetraose-containing LPS.{66907,66908} Cayman's LgtD (H. influenzae F3031, recombinant) protein can be used for enzyme activity assays.
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