Formulation: 100 mM Tris-HCl, pH 7.4, containing 0.1% BSA
Shelf life (days): 365
Notes: Each vial contains purified potato 5-lipoxygenase (5-LO) supplied in 0.1 M Tris-HCl (pH 7.4) containing 0.1% BSA. 5-LO has been formulated to be used for screening 5-LO specific inhibitors using the Lipoxygenase Inhibitor Screening Assay (Item No. 760700). There is enough enzyme for use in 96 wells. When following the Lipoxygenase Inhibitor Screening Assay protocol, a 90 µl aliquot of the diluted enzyme causes a final absorbance of 0.25-0.29 using linoleic acid as the substrate.