Formulation: 50 mM potassium phosphate, pH 7.6, with 10% glycerol
Purity: ≥90% estimated by SDS-PAGE
Shelf life (days): 365
Notes: NADPH cytochrome P450 reductase (POR) is a flavoprotein and key electron donor to cytochrome P450s (CYPs).{67523,67524} It is composed of an N-terminal membrane anchor domain and FMN-binding domain, which is linked to a C-terminal FAD-binding domain and NADPH-binding domain and localized to the cytosolic side of the endoplasmic reticulum.{67525,67523,67526} POR transfers electrons from NADPH through FAD and FMN coenzymes into the iron of the prosthetic heme group of CYPs.{67525,67523} Cortical levels of POR are increased in the APPswe/PSEN1dE9 transgenic mouse model of Alzheimer’s disease.{67527} Deficiency in POR is associated with ambiguous genitalia and the disruption of steroid metabolism in patients with Antley-Bixler syndrome.{67528} Cayman’s NADPH Cytochrome P450 Reductase (human, recombinant) can be used for enzyme activity assays.
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