Formulation: Lyophilized from sterile PBS, pH 7.4
Purity: ≥90% estimated by SDS-PAGE
Shelf life (days): 365
Notes: Palmitoyl-protein thioesterase 1 (PPT1) is a lysosomal hydrolase involved in removing palmitic acid (Item No. 10006627) from post-translationally modified proteins prior to their degradation.{65600} It is mainly expressed in the brain but is also found in visceral macrophages from liver, lung, and bowel tissues.{65601} PPT1 depalmitoylates proteins in cells undergoing autophagy, and PPT1 activity is important for axon outgrowth, neurite extension, and dendritic spine morphology.{65600} Knockout of PPT1 decreases tumor growth in a mouse xenograft model, and high expression of PPT1 is associated with poor overall survival in several cancers.{63265} Mutations in PPT1 cause infantile neuronal ceroid lipofuscinoses (INCL), an encephalopathy characterized by granular deposits in neurons, vision loss, seizures, mental deterioration, and brain death.{65602,65603} Cayman’s PPT1 (human, recombinant) protein consists of 290 amino acids, has a calculated molecular weight of 32.7 kDa, and a predicted N-terminus of Asp28 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass is 35-41 kDa due to glycosylation.
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