Formulation: 40 mM Tris-HCl, pH 8.0, with 110 mM sodium chloride, 2.2 mM potassium chloride, 80 µg/ml DYKDDDDK peptide, and 20% glycerol
Purity: ≥89% estimated by SDS-PAGE
Shelf life (days): 180
Notes: Protein-arginine N-methyltransferase 5 (PRMT5) is a type II PRMT and member of the class I S-adenosylmethionine-dependent methyltransferases that symmetrically dimethylates arginine residues in proteins and histones.{63086} It is composed of an N-terminal TIM domain and a C-terminal catalytic domain. PRMT5 proteins interact with methylosome 50 (MEP50) proteins primarily via the TIM domain to form a hetero-octameric complex that is more active than PRMT5 alone.{63087} Both PRMT5 and MEP50 are found in a wide variety of somatic and embryonic tissues, including the fetal ovary, fetal heart, and adult retina.{63088} The PRMT5-MEP50 complex methylates spliceosomal proteins and newly synthesized histone H2A in the cytoplasm and interacts with additional binding partners to regulate transcription through the methylation of NF-κB and p53 and other transcription factors in the nucleus. PRMT5 and MEP50 expression is increased in a variety of cancers and correlated with lower survival rates in patients with non-small cell lung cancer (NSCLC). Mutations in each gene are also associated with many types of cancer. Cayman's PRMT5-MEP50 protein can be used for enzyme activity assays.
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