Formulation: 25 mM Tris-HCl, pH 8.0, with 100 mM sodium chloride, 0.05% Tween 20, 50% glycerol, 10 mM reduced glutathione, and 3 mM DTT
Purity: ≥90% estimated by SDS-PAGE
Shelf life (days): 180
Notes: Rearranged during transfection (RET) is a receptor tyrosine kinase with roles in cell proliferation, survival, metabolism, and migration, as well as in the development of the peripheral and central nervous systems.{60954,60955} It is composed of an extracellular domain, containing four cadherin-like domains (CLDs), a calcium-binding site, and a cysteine-rich region, a transmembrane domain, and an intracellular kinase domain.{60954,60956} RET forms heterodimeric complexes with the glial cell line-derived neurotrophic factor (GDNF) ligands GDNF, neurturin (NRTN), artemin (ARTN), and persephin (PSPN), and the GDNF family co-receptors GFRα1-4, which induces autophosphorylation of the intracellular kinase domain and activates cellular signaling.{60956} Germline nonsense and/or missense mutations in RET decrease functional RET in gut tissue during development and are associated with Hirschsprung’s disease.{60954,60956} Loss-of-function mutations in RET are associated with congenital anomalies of the kidney and urinary tract (CAKUT) and renal agenesis.{60956} Gain-of-function mutations in RET are associated with medullary thyroid cancer, pheochromocytoma, and parathyroid hypoplasias, and RET gene fusions have been found in patients with lung, colon, or breast carcinomas.{60955,60956} Cayman’s RET Extracellular Domain (human, recombinant) protein can be used for enzyme activity assays.
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