Formulation: 20 mM HEPES, pH 7.5, with 2.5 mM DTT and 10% glycerol
Purity: ≥95% estimated by SDS-PAGE
Shelf life (days): 365
Notes: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped positive-stranded RNA virus and the causative agent of COVID-19, a primarily respiratory illness characterized by fever, cough, and shortness of breath that can lead to life-threatening complications.{53462,53459,53461,55257,55258} The SARS-CoV-2 genome contains approximately 30 kilobases and 14 open reading frames (ORFs) that encode four structural proteins: spike, envelope, membrane, and nucleocapsid, as well as 16 non-structural proteins and 9 accessory factors.{57176} The SARS-CoV-2 main protease (Mpro), also known as 3C-like protease (3CLpro), is encoded within the non-structural protein 5 (nsp5) region of ORF1ab.{57176} Auto-activation of SARS-CoV-2 Mpro cleaves nsp5-16, which, together with the nsp1-4, form the SARS-CoV-2 replication and transcription complex (RTC), which is critical for the viral replication cycle.{57176} Inhibition of SARS-CoV-2 Mpro activity reduces SARS-CoV-2 replication in infected cells in vitro, as well as decreases lung viral loads and pulmonary lesions in ACE2 transgenic mice infected with SARS-CoV-2.{59878,59879} Cayman's SARS-CoV-2 Mpro protein can be used for enzyme activity assays.
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