Formulation: 50 mM sodium acetate, pH 6.0, with 0.1% Triton X-100 and 1 mM calcium chloride
Purity: ≥50% estimated by SDS-PAGE
Shelf life (days): 365
Notes: Sphingolipid ceramide N-deacylase (SCDase) is an enzyme that catalyzes the hydrolysis or synthesis of the N-acyl linkage between the fatty acyl chain and sphingosine base in glycosphingolipids and sphingomyelin but not in free ceramide.{65694,18803} It has a broad substrate specificity for both hydrolysis and synthesis with preferences for glycosphingolipids with large sugar moieties and charged head groups for the hydrolysis reaction and glycosphingolipids with more polar head groups for the synthesis reaction. SCDase has been used as a biocatalyst for the synthesis and hydrolysis of glycosphingolipids.{65694,65695} Cayman’s SCDase (Shewanella alga, recombinant) protein can be used for bioconversion and enzyme activity assay applications.
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