Formulation: 50 mM sodium phosphate, pH 7.2, with 100 mM sodium chloride and 20% glycerol
Purity: ≥90% estimated by SDS-PAGE
Shelf life (days): 365
Notes: The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) and have been shown to catalyze a reaction that couples lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose from NAD+ and the abstracted acetyl group.{15271,15275,15273} There are seven human sirtuins, which have been designated SIRT1-7.{15274} SIRT2 is a cytoplasmic protein responsible for the deacetylation of histone H4 and a-tubulin, a modification important for controlling the cell cycle. Specifically, SIRT2 co-localizes with HDAC6 and microtubules and functions as a mitotic checkpoint in preventing chromosomal instability that can lead to hyperploid cells. SIRT2 is found in many tissues, but is specifically enriched in skeletal muscle, the heart, and in oligodendroglia cells in the brain.{17250,17251}
English
Deutsch
