Formulation: 50 mM sodium phosphate, pH 7.2, with 100 mM sodium chloride and 20% glycerol
Purity: ≥60% estimated by SDS-PAGE
Shelf life (days): 365
Notes: The sirtuins (SIRTs) represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) and have been shown to catalyze a reaction that couples lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose from NAD+ and the abstracted acetyl group.{15271,15275,15273} There are seven human SIRTs, which have been designated SIRT 1-7.{15274} SIRT3, is a mitochondrial protein, with its N-terminal 25 amino acid residues responsible for its localization.{15787,15788} Synthesized as an enzymatically inactive protein, human SIRT3 is activated by a matrix-processing peptidase.{15788} Recently, it was demonstrated that SIRT3 is translocated to the mitochondria from the nucleus during cellular stress or by the overexpression of SIRT3 itself.{15789} In mice, caloric restriction up-regulates SIRT3 expression levels in white and brown adipose tissue (WAT & BAT). Cold exposure also induces SIRT3 in brown adipose tissue (BAT).{15790} The constitutive expression of SIRT3 promotes the expression of PGC-1a, UCP1, and other genes involved in mitochondrial functions, indicating that SIRT3 modulates adaptive thermogenesis in BAT.{15790}
English
Deutsch
