Formulation: 50 mM NaPO4, pH 7.2, 100 mM sodium chloride, 5mM DTT, 20% glycerol
Purity: ≥85% estimated by SDS-PAGE
Shelf life (days): 730
Notes: Sirtuin 5 (SIRT5) is an enzyme that catalyzes the NAD-dependent removal of malonyl, succinyl, and glutaryl groups from target proteins.{55107,55108} Although it was originally characterized as a class III histone deacetylase (HDAC), SIRT5 exhibits weak NAD-dependent deacetylase activity in vitro and in vivo. SIRT5 is localized to mitochondria and is composed of a zinc binding domain and a Rossmann fold domain with the NAD and substrate binding sites located at the domain interface.{55107} Knockdown of Sirt5 induces hepatic and muscle protein hypersuccinylation, as well as fatty acid oxidation defects and hypertrophic cardiomyopathy in mice.{55109} SIRT5 protein and mRNA expression is elevated in human non-small cell lung cancer (NSCLC) tumors and is predictive of tumor recurrence and poor survival.{55110} SIRT5 also interacts with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) non-structural protein 14 (Nsp14), an exoribonuclease component of the viral replicase-transcriptase complex.{55024} Cayman’s SIRT5 (human, recombinant) protein can be used for ELISA and western blot applications.
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