Formulation: 50 mM Tris, pH 7.4, with 5% glycerol, 0.2 mM DTT, and 0.2 mM EDTA
Clone Designation: Slightly modified from wild-type
Purity: ≥90% estimated by SDS-PAGE
Shelf life (days): 180
Notes: Thromboxane A synthase (TXAS), also known as cytochrome P450 (CYP) isoform CYP5A1, is the enzyme that catalyzes the isomerization of prostaglandin H2 (PGH2; Item No. 17020) into thromboxane A2 (TXA2), a vasoconstrictor and an inducer of platelet aggregation.{61821,61822} It also catalyzes the cleavage of PGH2 into malondialdehyde (MDA) and 12(S)-hydroxyheptadecatrienoic acid (12(S)-HHT; Item No. 34590), a leukotriene B4 (LTB4) receptor 2 (BLT2) agonist that also has a role in platelet aggregation.{61821} TXAS exists as a monomer and is composed of an N-terminal membrane anchor domain, a heme-binding catalytic residue, and several substrate-binding residues.{61823,61824,61825} It is expressed in numerous cells, including platelets, monocytes, and macrophages, as well as several tissues, and is localized to the endoplasmic reticulum.{61823} TXAS undergoes suicide inactivation during catalysis. Mice deficient in TXAS exhibit prolonged bleeding time and defective platelet aggregation.{61826} Cayman's Thromboxane A Synthase (human, recombinant) protein can be used for enzyme activity assay and Western blot (WB) applications. To construct this protein, the N-terminal amino acids 1-29 of human CYP5A1 were removed and replaced with a hydrophilic sequence. The F-G loop of TXAS was modified using sequences from rabbit CYP2C5 and CYP2C3. The C-terminal of this protein contains 6x-His tag followed by a stop codon.
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