Formulation: 50 mM HEPES, pH 8.0, 150 mM sodium chloride, 1 mM DTT, and 0.1 mM EDTA
Purity: ≥95% estimated by SDS-PAGE
Shelf life (days): 365
Notes: Ubiquitin-specific protease 2 (USP2), also known as ubiquitin carboxyl-terminal hydrolase 2, is a cysteine protease involved in intracellular protein degradation by deubiquitinating degraded protein, including Mdm2, Mdm4, and CCND1.{40253,40254,40255} In both normal and cancer cells, it regulates G1/S cell-cycle transition.{40254} It may regulate p53-dependent pathways by deubiquitinating Mdm2, indirectly promoting p53 degradation and limiting its activity.{40253,40256,40257}. Regulated by androgen, USP2 is over-expressed in prostate cancer and stabilizes fatty acid synthase (FASN), which is associated with the malignancy of some aggressive prostate cancers.{40256,40258} Some of these oncogenic properties may be linked to its deubiquitinating activity. One hypothesis is that USP2 may stabilize short-lived proteins that may act to induce apoptosis.{40259} USP2 has also been shown to regulate the intrinsic circadian rhythm and its capacity to respond to external cues, in a non-degradative manner by acting on the core clock component PER1.{40260} This protein is a truncation containing the active catalytic domain (residues 259-605) of USP2 and a C-terminal His-tag.
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