Formulation: 50 mM HEPES, pH 8.0, with 150 mM sodium chloride and 10% glycerol
Purity: ≥85% estimated by SDS-PAGE
Shelf life (days): 365
Notes: YTH domain containing 2 (YTHDC2) is an ATP-dependent 3’-5’ RNA helicase and a member of the DExD/H-box family of helicases.{42792} YTHDC2 contains ATP binding, ATP hydrolysis, and RNA binding motifs.{42792} The YTH domain binds to N6-methyladenosine-modified germline messenger RNA to regulate meiotic gene expression in mammalian germ cells, mediating the mitotic-to-meiotic transition in mouse germ cells.{42795,42794} Ythdc2-/- male and female mice show disrupted meiotic prophase progression.{42794} Male Ythdc2-/- mice have degraded germ cells and lack mature spermatozoa, while female Ythdc2-/- mice lack developing ovarian follicles and have few germ cells. YTHDC2 also facilitates hepatitis C virus (HCV) genome replication by binding to the HCV replication cofactor cyclophilin B and the HCV RNA polymerase non-structural protein 5B (NS5B).{42793} YTHDC2 downregulation inhibits proliferation in Huh7 hepatocellular carcinoma cells and reduces metastasis a Y2KD-116 mouse xenograft model using HCT116 cells in which YTHDC2 is downregulated.{42793,35924} The expression of YTHDC2 in isolated human tumor tissue is positively correlated with tumor stage and lymph node metastasis.{35924}
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