Species: Arachis hypogaea
Product Description: Peanut lectin (PNA) is an identical tetrameric carbohydrate free protein with MW of 110 kDa. Thomsen-Friedenreich antigen, T-antigen (Galβ1, 3GalNAc) is present on blood group M &N glycoproteins (after removal of sialic acid with neuraminidase), glyconjugates (Mucin type), gangliosides and many glycolipids. T antigen is rarely expressed on normal coloncytes whereas cells of malignant, premalignant cells express this antigen. Peanut lectin has widely been used to detect T antigen in malignant and premalignant cells.
Application Note: WB: 1:500-1:2500. ELISA: 1:500-1:2500. IHC: 1:250-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Specificity/Sensitivity: Carbohydrate-Binding Specificity of Peanut Lectin : Galβ1, 3 GalNAc>>GalNAc>Gal
Form: Liquid
Buffer (with preservative): 10mM Bicarbonate, 150mM NaCl, 0.1mM Calcium Chloride (pH8.2). Dilute in PBS, 0.1mM Calcium Chloride, 0.05% Sodium azide.
Concentration: 5 mg/ml (Please refer to the vial label for the specific concentration.)
Background: Peanut lectin (PNA) is an identical tetrameric carbohydrate free protein with MW of 110 kDa. Thomsen-Friedenreich antigen, T-antigen (Galβ1, 3GalNAc) is present on blood group M &N glycoproteins (after removal of sialic acid with neuraminidase), glyconjugates (Mucin type), gangliosides and many glycolipids. T antigen is rarely expressed on normal coloncytes whereas cells of malignant, premalignant cells express this antigen. Peanut lectin has widely been used to detect T antigen in malignant and premalignant cells. Peanut lectin contains one atom of Ca++ and Mg++ per unit.
Inhibiting/Eluting sugars: 200 mM Galactose
Carbohydrate-Binding Specificity of PNA: Galβ1, 3 GalNAc>>>>>GalNAc>Gal
Region Sequence: Native Protein
ExpressionSystem: Native Protein
Purification: Purified by affinity chromatography followed by gel filtration chromatography
Conjugation: Biotin
Full Name: Peanut agglutinin lectin, Arachis hypogaea
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