Recombinant Cynomolgus Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-Fc)

Active protein: YES.

Activity: Immobilized Cynomolgus Siglec-15-Fc(Cat#PKSQ050112) at 2μg/ml (100 μl/well) can bind Anti-Human Siglec15 mAb. The ED50 of Anti-Human Siglec15 mAb is 20.9 ng/ml.

Protein Construction: Recombinant Cynomolgus Sialic Acid-binding Ig-like lectin 15 is produced by our Mammalian expression system and the target gene encoding Phe20-Thr263 is expressed with a Fc tag at the C-terminus.

Sequence: Phe20-Thr263.

Fusion tag: C-Fc.

Accession: A0A2K5UY47.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Mol Mass: 53.1 kDa.

AP Mol Mass: 55-70 kDa.

Formulation: Lyophilized from a 0.2 μm filtered solution of 50mM Tris-HCl, 100mM Glycine, 150mM NaCl, pH 7.5.

Background: Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO−-terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.

• SKU: ELSPKSQ050112-50
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: ELA-PKSQ050112-50
• Package Unit: 50 µg
• Quantity Unit: STK
• Reactivity: Cynomolgus Monkey (Primate)
• Application: ELISA
• Host: Human