Recombinant Human Galectin-3/LGALS3 Protein, Low Endotoxin

Protein Tag: N-His

Uniprot: P17931

Accession: P17931

Background: Leukotriene A-4 hydrolase; also known as LTA-4 hydrolase; Leukotriene A (4) hydrolase; LTA4H and LTA4; is cytoplasm protein which belongs to thepeptidase M1 family. LTA4H hydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). This enzyme also has some peptidase activity. The leukotrienes (LTs) are a class of structurally related lipid mediators involved in the development and maintenance of inflammatory and allergic reactions. In the biosynthesis of LTs; arachidonic acid was converted into the unstable intermediate epoxide LTA4; which may in turn be conjugated with glutathione to form the spasmogenic LTC4; or hydrolyzed into the proinflammatory lipid mediator LTB4 in a reaction catalyzed by Leukotriene A4 hydrolase (LTA4H). LTB4 is a classical chemoattractant of human neutrophils and triggers adherence and aggregation of leukocytes to vascular endothelium; and also modulates immune responses. As a bifunctional zinc metalloenzyme; LTA4H also exhibits an anion-dependant arginyl aminopeptidase activity of high efficiency and specificity in addition to its epoxide hydrolase activity. LTA4H is regarded as a therapeutic target for inflammation.

Bio Acitivity: Measured by its ability to chemoattract human PBMC using a concentration range of 2.5-25 μg/mL. Note: Results may vary from different PBMC donors.

Sequence: Ala 2-Ile 250

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 27 kDa

Observed MW: 32 kDa

• SKU: ELSPKSH031707-20
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: PKSH031707-20
• Package Unit: 20μg
• Quantity Unit: STK
• Reactivity: Human
• Application: Cell Culture
• Host: Escherichia Coli