Protein Construction: Recombinant Human Langerin is produced by our Mammalian expression system and the target gene encoding Tyr64-Pro328(Val278Ala) is expressed with a 6His tag at the N-terminus.
Sequence: Tyr64-Pro328(Val278Ala).
Fusion tag: N-His.
Accession: Q9UJ71.
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per μg as determined by the LAL method.
Mol Mass: 31.5 kDa.
AP Mol Mass: 30-40 kDa.
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Background: Langerin (CD207) is a type II transmembrane glycoprotein which is member K of the C-type lectin domain family. Langerin is used as a marker for Langerhans cells (LCs) which represent the immature dendritic cells in the epidermis. Langerin is necessary and sufficient for Birbeck granule formation. Human langerin sequence contains a 43 aa cytoplasmic domain, a 21 aa transmembrane domain and a 264 aa extracellular domain (ECD) that contains a coiled-coil domain and a single C-type lectin domain. Human langerin shares 68%, 62%, 71% aa identity with mouse, rat and bovine langerin ECD, respectively. Trimerization greatly increases the lectin binding affinity. Langerin internalizes endogenous proteins such as type I procollagen. Internalization by LC is thought to lead to suppression of self reactions. Langerin also mediates endocytosis of non-peptide antigens containing mannose, N-acetyl glucosamine and fucose that are expressed by mycobacteria and fungae.
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