Recombinant Human NKG2D/CD314 Protein (His Tag)(Active)

Active protein: YES.

Activity: Immobilized Human NKG2D-His at 10μg/ml(100 μl/well) can bind Human MICA-Fc(Cat: PKSH032753). The ED50 of Human NKG2D-His is 19.8 ug/ml .

Protein Construction: Recombinant Human NKG2-D type II Integral Membrane Protein is produced by our Mammalian expression system and the target gene encoding Phe78-Val216 is expressed with a 6His tag at the N-terminus.

Sequence: Phe78-Val216.

Fusion tag: N-6His.

Accession: P26718.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Mol Mass: 16.9 kDa.

AP Mol Mass: 20-30 kDa.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Background: NKG2-D type II integral membrane protein (NKG2D) is a type II transmembrane glycoprotein which belongs to the CD94/NKG2 family. NKG2D is expressed on natural killer (NK) cells; CD8+ alpha-beta and gamma-delta T-cells. As an activating and costimulatory receptor; it involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. It provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells; leading to cytotoxic activity. It stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx; culminating in the expression of TNF-alpha. NKG2D participates in NK cell-mediated bone marrow graft rejection and survival of NK cells. It Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including MICA; MICB; RAET1E; RAET1G; ULBP1; ULBP2; ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4.

• SKU: ELSPKSH033409-10
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: ELA-PKSH033409-10
• Green Labware: No
• Package Unit: 10 µg
• Quantity Unit: STK
• Reactivity: Human
• Application: ELISA
• Host: Human