Recombinant Human SIGLEC9/CD329 Protein (His Tag)(Active)

Active protein: YES.

Activity: Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Approximately 93% will adhere after 5-10 mins incubation at 37 °C.

Protein Construction: Recombinant Human SIGLEC9 is produced by our Mammalian expression system and the target gene encoding Gln18-Gly348(Lys100Glu;Ala315Glu) is expressed with a 6His tag at the C-terminus.

Sequence: Gln18-Gly348(Lys100Glu;Ala315Glu).

Fusion tag: C-His.

Accession: Q9Y336.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per μg as determined by the LAL method.

Mol Mass: 36.9 kDa.

AP Mol Mass: 55-90 kDa.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS，2mMEDTA，ph7.4.

Background: Sialic acid-binding Ig-like lectin 9(Siglec 9) is expressed by peripheral blood leukocytes (neutrophils and monocytes but not eosinophils); and found in liver; fetal liver; bone marrow; placenta; spleen and in lower levels in skeletal muscle; fetal brain and so on. It is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It also binds to alpha-2;3- or alpha-2;6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

• SKU: ELSPKSH033787-10
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: ELA-PKSH033787-10
• Green Labware: No
• Package Unit: 10 µg
• Quantity Unit: STK
• Reactivity: Human
• Application: ELISA, Cell Culture
• Host: Human