Notes: 7-keto-25-hydroxy Cholesterol is an oxysterol and a proposed metabolite of 7-keto cholesterol (Item No. 16339) and 7-dehydro cholesterol (Item No. 14612).{36976} It binds to and activates Smoothened (Smo) at the conserved extracellular cysteine-rich domain (CRD) in a reporter assay. It also increases EGF high affinity binding site numbers in normal rat kidney (NRK) cells.{36977}