Formulation: A solution in methyl acetate
Formal Name: N-(4-nitrophenyl)-5Z,8Z,11Z,14Z-eicosatetraenamide
Purity: ≥98%
Formula Markup: C26H36N2O3
Formula Weight: 424,6
Shelf life (days): 730
CAS Number: 119520-58-0
Notes: Arachidonoyl p-nitroaniline (ApNA) is one of several nitroaniline fatty acid amides which can be used to measure fatty acid amide hydrolase (FAAH) activity.{8928} FAAH is a relatively unselective enzyme in that it accepts a variety of amide head groups other than the ethanolamine of its nominal endogenous substrate anandamide (AEA; Item No. 90050). It also will hydrolyze fatty acid amides with fewer carbons and fewer double bonds than arachidonate. (See also Decanoyl p-Nitroaniline - Catalog No. 90349). Exposure of ApNA to FAAH activity results in the release of the yellow colorimetric dye p-nitroaniline (ε = 13,500 at 382 nm). This offers the potential for fast and convenient measurements of FAAH activity using a 96-well plate spectrophotometer.
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