Formulation: A solution in methanol at 100 µg/ml
Formal Name: (3α,5β,12α)-3,12-dihydroxy-cholan-24-oic-2,2,4,4-d4 acid
Purity: ≥95%
Formula Markup: C24H36D4O4
Formula Weight: 396,6
Shelf life (days): 1825
CAS Number: 112076-61-6
Notes: Deoxycholic acid-d4 (DCA-d4) is intended for use as an internal standard for the quantification of DCA (Item Nos. 20756 / 18231) by GC- or LC-MS. DCA is a secondary bile acid that is formed via microbial transformation of cholic acid (Item No. 20250) in the colon.{54006} It can be conjugated to glycine or taurine (Item No. 27031) to produce glycodeoxycholic acid (GDCA; Item No. 20274) or taurodeoxycholic acid (TDCA; Item No. 15935), respectively, in hepatocytes.{54006,33902,52692} DCA (0.2% v/v) inhibits spore germination induced by taurocholic acid (TCA; Item No. 16215) in seven C. difficile strains, as well as inhibits growth and decreases the cytotoxicity of C. difficile culture supernatants to Vero cells when used at a concentration of 0.02% v/v.{54006} It inhibits ionizing radiation-induced p53-dependent transcription in a reporter assay using HCT116 cells when used at a concentration of 200 µM.{52693} Fecal and intestinal tissue levels of DCA are increased in a rat model of high-fat diet-induced obesity compared with rats fed a normal diet.{54007} Increased serum DCA levels have been found in patients with colorectal cancer.{52694} DCA-d4 MaxSpec® standard is a quantitative grade standard of DCA-d4 (Item No. 20851) that has been prepared specifically for mass spectrometry and related applications where quantitative reproducibility is required. The solution has been prepared gravimetrically and is supplied in a deactivated glass ampule sealed under argon. The concentration was verified by comparison to an independently prepared calibration standard. The verified concentration is provided on the certificate of analysis. This DCA-d4 MaxSpec® standard is guaranteed to meet identity, purity, stability, and concentration specifications and is provided with a batch-specific certificate of analysis. Ongoing stability testing is performed to ensure the concentration remains accurate throughout the shelf life of the product. Note: The amount of solution added to the vial is in excess of the listed amount. Therefore, it is necessary to accurately measure volumes for preparation of calibration standards. Follow recommended storage and handling conditions to maintain product quality.
English
Deutsch
