Formulation: A crystalline solid
Formal Name: 1-O-hexadecanoyl-2-thio-R-(hexadecanoyl)-sn-glycerol-3-phosphocholine
Purity: ≥98%
Formula Markup: C40H80NO7PS
Formula Weight: 750,1
Shelf life (days): 730
CAS Number: 113881-60-0
Notes: Thioester analogs of glycerophospholipids, in combination with Ellman’s reagent, are convenient colorimetric substrates for the measurement of phospholipase (PL) activity.{1358,1359} Palmitoyl thio-PC is a chromogenic PLA2 substrate that contains a palmitoyl thioester at the sn-2 position of the glycerol backbone. Hydrolysis of the thioester by PLA2 yields a free thiol that reacts readily with DTNB (Ellman’s reagent) giving a bright yellow product with an absorbance maximum at 412 nm. Palmitoyl thio-PC has been used to measure bee venom sPLA2 activity in a phospholipid:Triton X-100 mixed micelle system.{15581}