Formulation: A solution in ethanol (1 μg/ml each compound)
Shelf life (days): 1825
Notes: This mixture contains the intermediary and secondary metabolites of prostaglandins (PGs) PGE2 (Item No. 14010), PGD2 (Item No. 12010), and PGF2α (Item No. 16010). The mixture is supplied in an amber ampule in which the headspace has been purged with argon to prevent lipid oxidation. This product has been designed for direct use in LC-MS applications. The solution may be serially diluted for preparation of calibrators and QC standards and/or used directly as a system suitability standard or tuning standard. After opening, we recommend that the mixture be transferred immediately to a 1 ml glass screw cap vial, to prevent solvent evaporation, and stored at -20°C. The mixture should be discarded after multiple freeze/thaw cycles.The tetranor-PG metabolites represented in this mixture are the major urinary metabolites of PGE2, PGD2, and PGF2α and, while biologically inactive, are used extensively as biomarkers to assess endogenous production of their respective parent PGs. 13,14-dihydro-15-keto PGs are intermediary metabolites of their respective parent PGs formed through the 15-hydroxy PGDH pathway. Though biologically active, these intermediary metabolites have relatively short half-lives and are virtually undetectable in urine.
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