Formulation: A solution in ethanol (100 ng/ml of each compound)
Shelf life (days): 1825
Notes: This mixture contains an E-series resolvin (specialized pro-resolving mediator; SPM) and its precursors, eicosapentaenoic acid (EPA; Item No. 90110) and (±)18-HEPE (Item No. 32840). The mixture is supplied in an amber ampule in which the headspace has been purged with argon to prevent lipid oxidation. This product has been designed for direct use in LC-MS applications. The solution may be serially diluted for preparation of calibrators and QC standards and/or used directly as a system suitability standard or tuning standard. After opening, we recommend that the mixture be transferred immediately to a 1 ml glass screw cap vial, to prevent solvent evaporation, and stored at -20°C. The mixture should be discarded after multiple freeze/thaw cycles.The fatty acids in this mixture represent the metabolic cascade of the E-series resolvins. Resolvin E1 (Item No. 10007848) has been postulated to be an anti-inflammatory metabolite of EPA by way of an 18-HEPE intermediate.{14504} It has been identified in mouse inflammatory exudates and in human plasma when subjects are treated with both aspirin (Item No. 70260) and supplemental EPA.{21162,11433,13210} Resolvin E1 has been shown to promote inflammatory resolution in numerous disease models, including experimental colitis, asthma, atherosclerosis, type 2 diabetes, and HSV-1-induced stromal keratitis.{13209,15876,30594,30592,30593,30595,30596}
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