Formulation: Lyophilized from sterile 20 mM Tris, pH 8.0, with 500 mM sodium chloride and 10% glycerol
Purity: ≥90% as estimated by SDS-PAGE
Formula Weight: 0
Shelf life (days): 365
Notes: Middle East respiratory syndrome coronavirus (MERS-CoV) is an enveloped positive-stranded RNA virus, a member of the Betacoronavirus genus, and a causative agent of MERS, an acute respiratory disease that often leads to pneumonia and renal failure.{62983,62985} The MERS-CoV nucleocapsid protein is composed of an intrinsically disordered region (IDR), an N-terminal domain that binds RNA, a flexible linker containing serine and arginine phosphorylation sites, a C-terminal domain that also participates in RNA binding and is responsible for dimerization, and another IDR.{51720,62925} Coronavirus nucleocapsid proteins, including the MERS-CoV nucleocapsid protein, package the viral genome into a ribonucleoprotein (RNP) complex.{62925} In addition, the MERS-CoV nucleocapsid protein interacts with the ubiquitin ligase TRIM25 and inhibits the activation of retinoic acid-inducible gene I (RIG-I) and phosphorylation of IFN regulatory factor 3 (IRF3) and NF-κB.{51721} It reduces IFN-β and IFN-λ1 promoter activity induced by the RIG-I caspase activation and recruitment domain (RIG-I-CARD), but not MDA5-CARD, and reduces Sendai virus-induced increases in IFN-β and IFN-λ1 mRNA levels. Cayman's MERS-CoV Nucleocapsid Protein (recombinant) consists of 424 amino acids and has a calculated molecular weight of 46.51 kDa.
English
Deutsch
