Alpha Synuclein pSer129 Pre-Formed Fibrils

Human Recombinant Alpha Synuclein pSer129 Pre-formed Fibrils

Target: Alpha Synuclein pSer129

Nature: Recombinant

Swiss-Prot: P37840

Expression System: E. coli

Protein Length: 140 aa

Amino Acid Sequence: MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA

Purification: Ion-exchange Purified

Purity: >95%

Storage Buffer: PBS pH 7.4

Protein Size: 14.46 kDa

Conjugate: No Tag

Scientific Background: Serine 129 is the C-terminal serine characteristic to mammalian alpha synuclein, with this serine being determined to be a major phosphorylation site (1). Lewy Bodies in Parkinson’s Disease (PD) and other related synucleinopathies are comprised of alpha synuclein phosphorylated at serine 129 and this phosphorylation may contribute to an increased propensity to aggregate (2). Due to phosphorylation at serine 129 being one of the most abundant PTMs, several studies reported on the PTM as a potential biomarker (3). Our Alpha Synuclein Ser129 Pre-Formed Fibrils are generated in-vitro from purified phosphorylated monomer and phosphorylation is confirmed with our anti-ASYN pS129 monoclonal antibody (Catalog# SMC-600).

References: 1. Okochi et al. 2000. Constitutive phosphorylation of the Parkinson’s Disease Associated a-Synuclein. The Journal of Biological Chemistry. DOI: 10.1074/jbc.275.1.3902. Fujiwara et al. 2002. α-Synuclein is phosphorylated in synucleinopathy lesions. Nature Cell Biology. DOI: 10.1038/ncb7483. Magalhaes and Lashuel. 2002. Opportunities and challenges of alpha-synuclein as a potential biomarker for Parkinson’s disease and other synucleinopathies. Npj Parkinsons Disease. DOI: 10.1038/s41531-022-00357-0

Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• SKU: STRSPR-521XG
• Manufacturer: Stressmarq Biosciences
• Manufacturer SKU: SPR-521XG
• Green Labware: No
• Package Unit: 500 µg x2 (@5mg/ml)
• Quantity Unit: PAK
• Reactivity: Human
• Application: Western Blotting, In Vivo Assay, SDS-PAGE